8fef

Structure of Mce1 transporter from Mycobacterium smegmatis (Map0)

spinqualitylabelsall models 8fef, resolution 2.71Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8fef is a 10 chain structure with sequence from Mycolicibacterium smegmatis MC2 155. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 2.71Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0QNR2_MYCS2 Publication Abstract from PubMed To replicate inside macrophages and cause tuberculosis, Mycobacterium tuberculosis must scavenge a variety of nutrients from the host(1,2). The mammalian cell entry (MCE) proteins are important virulence factors in M. tuberculosis(1,3), where they are encoded by large gene clusters and have been implicated in the transport of fatty acids(4-7) and cholesterol(1,4,8) across the impermeable mycobacterial cell envelope. Very little is known about how cargos are transported across this barrier, and it remains unclear how the approximately ten proteins encoded by a mycobacterial mce gene cluster assemble to transport cargo across the cell envelope. Here we report the cryo-electron microscopy (cryo-EM) structure of the endogenous Mce1 lipid-import machine of Mycobacterium smegmatis-a non-pathogenic relative of M. tuberculosis. The structure reveals how the proteins of the Mce1 system assemble to form an elongated ABC transporter complex that is long enough to span the cell envelope. The Mce1 complex is dominated by a curved, needle-like domain that appears to be unrelated to previously described protein structures, and creates a protected hydrophobic pathway for lipid transport across the periplasm. Our structural data revealed the presence of a subunit of the Mce1 complex, which we identified using a combination of cryo-EM and AlphaFold2, and name LucB. Our data lead to a structural model for Mce1-mediated lipid import across the mycobacterial cell envelope. Structure of an endogenous mycobacterial MCE lipid transporter.,Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert DC Nature. 2023 Aug;620(7973):445-452. doi: 10.1038/s41586-023-06366-0. Epub 2023 , Jul 26. PMID:37495693 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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