8g6b
Crystal structure of PfAMA1-RON2L chimera
Structural highlights
Publication Abstract from PubMedInvasion of human erythrocytes by Plasmodium falciparum (Pf) merozoites relies on the interaction between two parasite proteins: apical membrane antigen 1 (AMA1) and rhoptry neck protein 2 (RON2). While antibodies to AMA1 provide limited protection against Pf in non-human primate malaria models, clinical trials using recombinant AMA1 alone (apoAMA1) yielded no protection due to insufficient functional antibodies. Immunization with AMA1 bound to RON2L, a 49-amino acid peptide from its ligand RON2, has shown superior protection by increasing the proportion of neutralizing antibodies. However, this approach relies on the formation of a complex in solution between the two vaccine components. To advance vaccine development, here we engineered chimeric antigens by replacing the AMA1 DII loop, displaced upon ligand binding, with RON2L. Structural analysis confirmed that the fusion chimera (Fusion-F(D12)) closely mimics the binary AMA1-RON2L complex. Immunization studies in female rats demonstrated that Fusion-F(D12) immune sera, but not purified IgG, neutralized vaccine-type parasites more efficiently compared to apoAMA1, despite lower overall anti-AMA1 titers. Interestingly, Fusion-F(D12) immunization enhanced antibodies targeting conserved epitopes on AMA1, leading to increased neutralization of non-vaccine type parasites. Identifying these cross-neutralizing antibody epitopes holds promise for developing an effective, strain-transcending malaria vaccine. Structure guided mimicry of an essential P. falciparum receptor-ligand complex enhances cross neutralizing antibodies.,Yanik S, Venkatesh V, Parker ML, Ramaswamy R, Diouf A, Sarkar D, Miura K, Long CA, Boulanger MJ, Srinivasan P Nat Commun. 2023 Sep 21;14(1):5879. doi: 10.1038/s41467-023-41636-5. PMID:37735574[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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