8h3w

Crystal structure of chicken egg lysozyme at ambient temperature

spinqualitylabelsall models 8h3w, resolution 2.39Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8h3w is a 1 chain structure with sequence from Gallus gallus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.39Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function LYSC_CHICK Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus. Publication Abstract from PubMed High-resolution biomacromolecular structure determination is essential to better understand protein function and dynamics. Serial crystallography is an emerging structural biology technique which has fundamental limitations due to either sample volume requirements or immediate access to the competitive X-ray beamtime. Obtaining a high volume of well-diffracting, sufficient-size crystals while mitigating radiation damage remains a critical bottleneck of serial crystallography. As an alternative, we introduce the plate-reader module adapted for using a 72-well Terasaki plate for biomacromolecule structure determination at a convenience of a home X-ray source. We also present the first ambient temperature lysozyme structure determined at the Turkish light source (Turkish DeLight). The complete dataset was collected in 18.5 min with resolution extending to 2.39 A and 100% completeness. Combined with our previous cryogenic structure (PDB ID: 7Y6A), the ambient temperature structure provides invaluable information about the structural dynamics of the lysozyme. Turkish DeLight provides robust and rapid ambient temperature biomacromolecular structure determination with limited radiation damage. Rapid and efficient ambient temperature X-ray crystal structure determination at Turkish Light Source.,Gul M, Ayan E, Destan E, Johnson JA, Shafiei A, Kepceoglu A, Yilmaz M, Ertem FB, Yapici I, Tosun B, Baldir N, Tokay N, Nergiz Z, Karakadioglu G, Paydos SS, Kulakman C, Ferah CK, Guven O, Atalay N, Akcan EK, Cetinok H, Arslan NE, Sabanoglu K, Asci B, Tavli S, Gumusboga H, Altuntas S, Otsuka M, Fujita M, Teki N S, Ci Ftci H, Durdagi S, Karaca E, Kaplan Turkoz B, Kabasakal BV, Kati A, DeMi Rci H Sci Rep. 2023 May 19;13(1):8123. doi: 10.1038/s41598-023-33989-0. PMID:37208392 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Lysozyme 3D structures

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