8h62

Crystal structure of Internalin A from Listeria monocytogenes with human E-cadherin EC12

spinqualitylabelsall models 8h62, resolution 1.91Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8h62 is a 2 chain structure with sequence from Homo sapiens and Listeria monocytogenes EGD-e. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.91Å Ligands: ACT, CA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function INLA_LISMO Mediates the entry of L.monocytogenes into cells. Publication Abstract from PubMed Listeriosis, caused by infection with Listeria monocytogenes, is a severe disease with a high mortality rate. The L. monocytogenes virulence factor, internalin family protein InlA, which binds to the host receptor E-cadherin, is necessary to invade host cells. Here, we isolated two single-domain antibodies (V(H)Hs) that bind to InlA with picomolar affinities from an alpaca immune library using the phage display method. These InlA-specific V(H)Hs inhibited the binding of InlA to the extracellular domains of E-cadherin in vitro as shown by biophysical interaction analysis. Furthermore, we determined that the V(H)Hs inhibited the invasion of L. monocytogenes into host cells in culture. High-resolution X-ray structure analyses of the complexes of V(H)Hs with InlA revealed that the V(H)Hs bind to the same binding site as E-cadherin against InlA. We conclude that these V(H)Hs have the potential for use as drugs to treat listeriosis. Anti-InlA single-domain antibodies that inhibit the cell invasion of Listeria monocytogenes.,Yamazaki T, Nagatoishi S, Yamawaki T, Nozawa T, Matsunaga R, Nakakido M, Caaveiro JMM, Nakagawa I, Tsumoto K J Biol Chem. 2023 Oct;299(10):105254. doi: 10.1016/j.jbc.2023.105254. Epub 2023 , Sep 14. PMID:37716701 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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