8h8a | pdb_00008h8a

Type VI secretion system effector RhsP in its post-autoproteolysis and monomeric form

spinqualitylabelsall models 8h8a, resolution 3.25Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8h8a is a 2 chain structure with sequence from Vibrio parahaemolyticus RIMD 2210633. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.25Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q87PI5_VIBPA Publication Abstract from PubMed The rearrangement hotspot (Rhs) repeat is an ancient giant protein fold found in all domains of life. Rhs proteins are polymorphic toxins that could either be deployed as an ABC complex or via a type VI secretion system (T6SS) in interbacterial competitions. To explore the mechanism of T6SS-delivered Rhs toxins, we used the gastroenteritis-associated Vibrio parahaemolyticus as a model organism and identified an Rhs toxin-immunity pair, RhsP-RhsP(I). Our data show that RhsP-dependent prey targeting by V. parahaemolyticus requires T6SS2. RhsP can bind to VgrG2 independently without a chaperone and spontaneously self-cleaves into three fragments. The toxic C-terminal fragment (RhsP(C)) can bind to VgrG2 via a VgrG2-interacting region (VIR). Our electron microscopy (EM) analysis reveals that the VIR is encapsulated inside the Rhs beta barrel structure and that autoproteolysis triggers a dramatic conformational change of the VIR. This alternative VIR conformation promotes RhsP dimerization, which significantly contributes to T6SS2-mediated prey targeting by V. parahaemolyticus. Vibrio parahaemolyticus prey targeting requires autoproteolysis-triggered dimerization of the type VI secretion system effector RhsP.,Tang L, Dong S, Rasheed N, Wu HW, Zhou N, Li H, Wang M, Zheng J, He J, Chao WCH Cell Rep. 2022 Dec 6;41(10):111732. doi: 10.1016/j.celrep.2022.111732. PMID:36476863 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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