8h9y

Crystal structure of voltage-gated sodium channel NavAb N49K/L176Q mutant in calcium ion condition

spinqualitylabelsall models 8h9y, resolution 3.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8h9y is a 1 chain structure with sequence from Aliarcobacter butzleri. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.4Å Ligands: 1N7, CA, LMT, PX4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A8EVM5_ALIB4 Publication Abstract from PubMed Divalent cation block is observed in various tetrameric ion channels. For blocking, a divalent cation is thought to bind in the ion pathway of the channel, but such block has not yet been directly observed. So, the behaviour of these blocking divalent cations remains still uncertain. Here, we elucidated the mechanism of the divalent cation block by reproducing the blocking effect into NavAb, a well-studied tetrameric sodium channel. Our crystal structures of NavAb mutants show that the mutations increasing the hydrophilicity of the inner vestibule of the pore domain enable a divalent cation to stack on the ion pathway. Furthermore, non-equilibrium molecular dynamics simulation showed that the stacking calcium ion repel sodium ion at the bottom of the selectivity filter. These results suggest the primary process of the divalent cation block mechanism in tetrameric cation channels. The structural basis of divalent cation block in a tetrameric prokaryotic sodium channel.,Irie K, Oda Y, Sumikama T, Oshima A, Fujiyoshi Y Nat Commun. 2023 Jul 15;14(1):4236. doi: 10.1038/s41467-023-39987-0. PMID:37454189 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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