8ho0

Crystal structure of cytochrome P450 NasF5053 mutant E73S complexed with 8FCWP

spinqualitylabelsall models 8ho0, resolution 1.71Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8ho0 is a 1 chain structure with sequence from Streptomyces sp. NRRL F-5053. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.71Å Ligands: 3ZI, CA, HEM Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A8I3B027_9ACTN Publication Abstract from PubMed Heterodimeric tryptophan-containing diketopiperazines (HTDKPs) are an important class of bioactive secondary metabolites. Biosynthesis offers a practical opportunity to access their bioactive structural diversity, however, it is restricted by the limited substrate scopes of the HTDKPs-forming P450 dimerases. Herein, by genome mining and investigation of the sequence-product relationships, we unveiled three important residues (F387, F388 and E73) in these P450s that are pivotal for selecting different diketopiperazine (DKP) substrates in the upper binding pocket. Engineering these residues in Nas(F5053) significantly expanded its substrate specificity and enabled the collective biosynthesis, including 12 self-dimerized and at least 81 cross-dimerized HTDKPs. Structural and molecular dynamics analysis of F387G and E73S revealed that they control the substrate specificity via reducing steric hindrance and regulating substrate tunnels, respectively. Engineering the Substrate Specificity of a P450 Dimerase Enables the Collective Biosynthesis of Heterodimeric Tryptophan-Containing Diketopiperazines.,Sun C, Ma BD, Li G, Tian W, Yang L, Peng H, Lin Z, Deng Z, Kong XD, Qu X Angew Chem Int Ed Engl. 2023 Jun 19;62(25):e202304994. doi: , 10.1002/anie.202304994. Epub 2023 May 8. PMID:37083030 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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