8htg
Crystal structure of Golf in complex with GTP-gamma S and Mg
Structural highlights
FunctionGNAL_MOUSE Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(olf) alpha mediates signal transduction within the olfactory neuroepithelium and the basal ganglia. May be involved in some aspect of visual transduction, and in mediating the effect of one or more hormones/neurotransmitters (By similarity). Publication Abstract from PubMedStructural and mechanistic studies on human odorant receptors (ORs), key in olfactory signaling, are challenging because of their low surface expression in heterologous cells. The recent structure of OR51E2 bound to propionate provided molecular insight into odorant recognition, but the lack of an inactive OR structure limited understanding of the activation mechanism of ORs upon odorant binding. Here, we determined the cryo-electron microscopy structures of consensus OR52 (OR52(cs)), a representative of the OR52 family, in the ligand-free (apo) and octanoate-bound states. The apo structure of OR52(cs) reveals a large opening between transmembrane helices (TMs) 5 and 6. A comparison between the apo and active structures of OR52(cs) demonstrates the inward and outward movements of the extracellular and intracellular segments of TM6, respectively. These results, combined with molecular dynamics simulations and signaling assays, shed light on the molecular mechanisms of odorant binding and activation of the OR52 family. Understanding the molecular mechanisms of odorant binding and activation of the human OR52 family.,Choi C, Bae J, Kim S, Lee S, Kang H, Kim J, Bang I, Kim K, Huh WK, Seok C, Park H, Im W, Choi HJ Nat Commun. 2023 Dec 7;14(1):8105. doi: 10.1038/s41467-023-43983-9. PMID:38062020[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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