8htz

E. coli 70S ribosome complexed with H. marismortui tRNA_Ile2 bearing agm2C34 in classical state

spinqualitylabelsall models 8htz, resolution 2.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8htz is a 10 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 2.4Å Ligands: 1MG, 2MA, 2MG, 3TD, 4D4, 4OC, 5MC, 5MU, 6MZ, 7S3, 7SN, B8H, D2T, G7M, H2U, IAS, K, M2G, MA6, MEQ, MG, MS6, OBX, OMC, OMG, OMU, PSU, T6A, U8U, UR3 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RS2_ECOLI Publication Abstract from PubMed The anticodon modifications of transfer RNAs (tRNAs) finetune the codon recognition on the ribosome for accurate translation. Bacteria and archaea utilize the modified cytidines, lysidine (L) and agmatidine (agm(2)C), respectively, in the anticodon of tRNA(Ile) to decipher AUA codon. L and agm(2)C contain long side chains with polar termini, but their functions remain elusive. Here we report the cryogenic electron microscopy structures of tRNAs(Ile) recognizing the AUA codon on the ribosome. Both modifications interact with the third adenine of the codon via a unique C-A geometry. The side chains extend toward 3' direction of the mRNA, and the polar termini form hydrogen bonds with 2'-OH of the residue 3'-adjacent to the AUA codon. Biochemical analyses demonstrated that AUA decoding is facilitated by the additional interaction between the polar termini of the modified cytidines and 2'-OH of the fourth mRNA residue. We also visualized cyclic N(6)-threonylcarbamoyladenosine (ct(6)A), another tRNA modification, and revealed a molecular basis how ct(6)A contributes to efficient decoding. Structural insights into the decoding capability of isoleucine tRNAs with lysidine and agmatidine.,Akiyama N, Ishiguro K, Yokoyama T, Miyauchi K, Nagao A, Shirouzu M, Suzuki T Nat Struct Mol Biol. 2024 May;31(5):817-825. doi: 10.1038/s41594-024-01238-1. , Epub 2024 Mar 27. PMID:38538915 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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