8iev

Crystal structure of the DUF2891 family protein CJ0554 from Campylobacter jejuni in space group C2

spinqualitylabelsall models 8iev, resolution 2.08Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8iev is a 6 chain structure with sequence from Campylobacter jejuni. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.08Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q0PAW2_CAMJE Publication Abstract from PubMed Campylobacter jejuni is a pathogenic bacterium that causes enteritis and Guillain-Barre syndrome in humans. To identify a protein target for the development of a new therapeutic against C. jejuni infection, each gene product of C. jejuni must be functionally characterized. The cj0554 gene of C. jejuni encodes a DUF2891 family protein with unknown functions. To provide functional insights into CJ0554, we determined and analyzed the crystal structure of the CJ0554 protein. CJ0554 adopts an (alpha/alpha)(6)-barrel structure, which consists of an inner alpha(6) ring and an outer alpha(6) ring. CJ0554 assembles into a dimer in a unique top-to-top orientation that is not observed in its structural homologs, N-acetylglucosamine 2-epimerase superfamily members. Dimer formation was verified by analyzing CJ0554 and its ortholog protein through gel-filtration chromatography. The top of the CJ0554 monomer barrel harbors a cavity, which is connected to that of the second subunit in the dimer structure, generating a larger intersubunit cavity. This elongated cavity accommodates extra nonproteinaceous electron density, presumably as a pseudosubstrate, and is lined with generally catalytically active histidine residues that are invariant in CJ0554 orthologs. Therefore, we propose that the cavity functions as the active site of CJ0554. Unique dimeric structure of the DUF2891 family protein CJ0554 from Campylobacter jejuni.,Kim SY, Cho HY, Yoon SI Biochem Biophys Res Commun. 2023 May 7;655:11-17. doi: , 10.1016/j.bbrc.2023.03.010. Epub 2023 Mar 6. PMID:36913761 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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