8ii8

X-ray crystal structure of pink-colored protein from Pleurotus salmoneostramineus in complex with natural chromophore

spinqualitylabelsall models 8ii8, resolution 1.25Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8ii8 is a 1 chain structure with sequence from Pleurotus salmoneostramineus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.25Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A2Z5U5S2_9AGAR Publication Abstract from PubMed The pink-colored protein from the fungus Pleurotus salmoneostramineus (PsPCP) possesses unusual primary sequences with little resemblance to those of known proteins and exhibits a red color in aqueous solution. To understand the pigmentation mechanism of PsPCP, we elucidated the X-ray crystal structure of the native PsPCP. We identified a highly conjugated polyene ligand 2-dehydro-3-deoxylaetiporic acid A as a chromophore ligand, whose solution exhibits yellow. The crystal structure of PsPCP indicated that the ligand is secured in the central cavity and anchored at both termini by hydrophilic interactions and that surrounding residues show CH-pi and C-H...O hydrogen bondings. Geometrical analyses of the bound ligand demonstrated that the conjugated C-C and C horizontal lineC bonds exhibit similar bond distances. The result indicated enhanced electron delocalization within the conjugated CC bond system, resulting in a redshift of the chromophore ligand. The computational estimates of the UV-vis spectra support the view that the electron delocalization within the conjugated CC bonds system of the bound ligand, induced by the specific ligand geometry within a limited space of PsPCP cavity, is responsible for the red pigmentation of PsPCP. Thus, we propose that the coloring mechanism of PsPCP, which constrains the geometry of a highly conjugated polyene ligand, is a novel type of pigment chemistry. Crystal Structure of the Native Chromoprotein from Pleurotus salmoneostramineus Provides Insights into the Pigmentation Mechanism.,Ihara M, Tsuchida N, Sumida M, Himiyama T, Kitayama T, Shirasaka N, Fukuta Y J Agric Food Chem. 2024 Aug 7;72(31):17626-17632. doi: 10.1021/acs.jafc.4c02951. , Epub 2024 Jul 29. PMID:39073883 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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