8ikw

A complex structure of PGIP-PG

spinqualitylabelsall models 8ikw, resolution 1.94Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8ikw is a 8 chain structure with sequence from Fusarium phyllophilum and Phaseolus vulgaris. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.94Å Ligands: BMA, FUC, MAN, NAG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Polygalacturonase-inhibiting proteins (PGIPs) interact with pathogen-derived polygalacturonases to inhibit their virulence-associated plant cell wall-degrading activity but stimulate immunity-inducing oligogalacturonide production. Here we show that interaction between Phaseolus vulgaris PGIP2 (PvPGIP2) and Fusarium phyllophilum polygalacturonase (FpPG) enhances substrate binding, resulting in inhibition of the enzyme activity of FpPG. This interaction promotes FpPG-catalyzed production of long-chain immunoactive oligogalacturonides, while diminishing immunosuppressive short oligogalacturonides. PvPGIP2 binding creates a substrate binding site on PvPGIP2-FpPG, forming a new polygalacturonase with boosted substrate binding activity and altered substrate preference. Structure-based engineering converts a putative PGIP that initially lacks FpPG-binding activity into an effective FpPG-interacting protein. These findings unveil a mechanism for plants to transform pathogen virulence activity into a defense trigger and provide proof of principle for engineering PGIPs with broader specificity. A plant mechanism of hijacking pathogen virulence factors to trigger innate immunity.,Xiao Y, Sun G, Yu Q, Gao T, Zhu Q, Wang R, Huang S, Han Z, Cervone F, Yin H, Qi T, Wang Y, Chai J Science. 2024 Feb 16;383(6684):732-739. doi: 10.1126/science.adj9529. Epub 2024 , Feb 15. PMID:38359129 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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