8in3

Eisenia hydrolysis-enhancing protein from Aplysia kurodai

spinqualitylabelsall models 8in3, resolution 1.15Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8in3 is a 1 chain structure with sequence from Aplysia kurodai. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.15Å Ligands: GOL Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A1B4XTR1_APLKU Publication Abstract from PubMed The defensive-offensive associations between algae and herbivores determine marine ecology. Brown algae utilize phlorotannin as their chemical defense against the predator Aplysia kurodai, which uses beta-glucosidase (akuBGL) to digest the laminarin in algae into glucose. Moreover, A. kurodai employs Eisenia hydrolysis-enhancing protein (EHEP) as an offense to protect akuBGL activity from phlorotannin inhibition by precipitating phlorotannin. To underpin the molecular mechanism of this digestive-defensive-offensive system, we determined the structures of the apo and tannic acid (TNA, a phlorotannin analog) bound forms of EHEP, as well as the apo akuBGL. EHEP consisted of three peritrophin-A domains arranged in a triangular shape and bound TNA in the center without significant conformational changes. Structural comparison between EHEP and EHEP-TNA led us to find that EHEP can be resolubilized from phlorotannin precipitation at an alkaline pH, which reflects a requirement in the digestive tract. akuBGL contained two GH1 domains, only one of which conserved the active site. Combining docking analysis, we propose the mechanisms by which phlorotannin inhibits akuBGL by occupying the substrate-binding pocket, and EHEP protects akuBGL against this inhibition by binding with phlorotannin to free the akuBGL pocket. Structural basis of EHEP-mediated offense against phlorotannin-induced defense from brown algae to protect akuBGL activity.,Sun X, Ye Y, Sakurai N, Wang H, Kato K, Yu J, Yuasa K, Tsuji A, Yao M Elife. 2023 Nov 1;12:RP88939. doi: 10.7554/eLife.88939. PMID:37910430 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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