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Yeast replisome in state I
Structural highlights
FunctionMCM4_YEAST Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Once loaded onto DNA, double hexamers can slide on dsDNA in the absence of ATPase activity. Required for S phase execution.[1] [2] Publication Abstract from PubMedThe replisome that replicates the eukaryotic genome consists of at least three engines: the Cdc45-MCM-GINS (CMG) helicase that separates duplex DNA at the replication fork and two DNA polymerases, one on each strand, that replicate the unwound DNA. Here, we determined a series of cryo-electron microscopy structures of a yeast replisome comprising CMG, leading-strand polymerase Polepsilon and three accessory factors on a forked DNA. In these structures, Polepsilon engages or disengages with the motor domains of the CMG by occupying two alternative positions, which closely correlate with the rotational movement of the single-stranded DNA around the MCM pore. During this process, the polymerase remains stably coupled to the helicase using Psf1 as a hinge. This synergism is modulated by a concerted rearrangement of ATPase sites to drive DNA translocation. The Polepsilon-MCM coupling is not only required for CMG formation to initiate DNA replication but also facilitates the leading-strand DNA synthesis mediated by Polepsilon. Our study elucidates a mechanism intrinsic to the replisome that coordinates the activities of CMG and Polepsilon to negotiate any roadblocks, DNA damage, and epigenetic marks encountered during translocation along replication forks. Synergism between CMG helicase and leading strand DNA polymerase at replication fork.,Xu Z, Feng J, Yu D, Huo Y, Ma X, Lam WH, Liu Z, Li XD, Ishibashi T, Dang S, Zhai Y Nat Commun. 2023 Sep 20;14(1):5849. doi: 10.1038/s41467-023-41506-0. PMID:37730685[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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