8kgq

Structure of African swine fever virus topoisomerase II in complex with dsDNA

spinqualitylabelsall models 8kgq, resolution 5.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8kgq is a 4 chain structure with sequence from African swine fever virus and DNA molecule. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 5.6Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A2X0THW2_ASF Publication Abstract from PubMed Type II topoisomerases are ubiquitous enzymes that play a pivotal role in modulating the topological configuration of double-stranded DNA. These topoisomerases are required for DNA metabolism and have been extensively studied in both prokaryotic and eukaryotic organisms. However, our understanding of virus-encoded type II topoisomerases remains limited. One intriguing example is the African swine fever virus, which stands as the sole mammalian-infecting virus encoding a type II topoisomerase. In this work, we use several approaches including cryo-EM, X-ray crystallography, and biochemical assays to investigate the structure and function of the African swine fever virus type II topoisomerase, pP1192R. We determine the structures of pP1192R in different conformational states and confirm its enzymatic activity in vitro. Collectively, our results illustrate the basic mechanisms of viral type II topoisomerases, increasing our understanding of these enzymes and presenting a potential avenue for intervention strategies to mitigate the impact of the African swine fever virus. Structural insights into the DNA topoisomerase II of the African swine fever virus.,Cong J, Xin Y, Kang H, Yang Y, Wang C, Zhao D, Li X, Rao Z, Chen Y Nat Commun. 2024 May 30;15(1):4607. doi: 10.1038/s41467-024-49047-w. PMID:38816407 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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