8onw

Crystal structure of the hetero-dimeric complex from Archaeoglobus fulgidus PRC1 and PRC2 domains

spinqualitylabelsall models 8onw, resolution 2.29Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8onw is a 8 chain structure with sequence from Archaeoglobus fulgidus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.29Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A117KMF0_ARCFL Publication Abstract from PubMed Cell division in all domains of life requires the orchestration of many proteins, but in Archaea most of the machinery remains poorly characterized. Here we investigate the FtsZ-based cell division mechanism in Haloferax volcanii and find proteins containing photosynthetic reaction centre (PRC) barrel domains that play an essential role in archaeal cell division. We rename these proteins cell division protein B 1 (CdpB1) and CdpB2. Depletions and deletions in their respective genes cause severe cell division defects, generating drastically enlarged cells. Fluorescence microscopy of tagged FtsZ1, FtsZ2 and SepF in CdpB1 and CdpB2 mutant strains revealed an unusually disordered divisome that is not organized into a distinct ring-like structure. Biochemical analysis shows that SepF forms a tripartite complex with CdpB1/2 and crystal structures suggest that these two proteins might form filaments, possibly aligning SepF and the FtsZ2 ring during cell division. Overall our results indicate that PRC-domain proteins play essential roles in FtsZ-based cell division in Archaea. Proteins containing photosynthetic reaction centre domains modulate FtsZ-based archaeal cell division.,Nussbaum P, Kureisaite-Ciziene D, Bellini D, van der Does C, Kojic M, Taib N, Yeates A, Tourte M, Gribaldo S, Loose M, Lowe J, Albers SV Nat Microbiol. 2024 Mar;9(3):698-711. doi: 10.1038/s41564-024-01600-5. Epub 2024 , Mar 4. PMID:38443575 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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