8osi

Genetically encoded green ratiometric calcium indicator FNCaMP in calcium-bound state

spinqualitylabelsall models 8osi, resolution 2.42Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8osi is a 1 chain structure with sequence from Aureobasidium namibiae CBS 147.97 and Branchiostoma lanceolatum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.42Å Ligands: CA, CR2 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A1S4NYF2_BRALA A0A254U4M1_ASPNG A0A100IBH9_ASPNG Publication Abstract from PubMed Neurobiologists widely use green genetically encoded calcium indicators (GECIs) for visualization of neuronal activity. Among them, ratiometric GECIs allow imaging of both active and non-active neuronal populations. However, they are not popular, since their properties are inferior to intensiometric GCaMP series of GECIs. The most characterized and developed ratiometric green GECI is FGCaMP7. However, the dynamic range and sensitivity of its large Stock's shift green (LSS-Green) form is significantly lower than its Green form and its molecular design is not optimal. To address these drawbacks, we engineered a ratiometric green calcium indicator, called FNCaMP, which is based on bright mNeonGreen protein and calmodulin from A. niger and has optimal NTnC-like design. We compared the properties of the FNCaMP and FGCaMP7 indicators in vitro, in mammalian cells, and in neuronal cultures. Finally, we obtained and analyzed X-ray structure of the FNCaMP indicator. FNCaMP, ratiometric green calcium indicator based on mNeonGreen protein.,Subach OM, Varfolomeeva L, Vlaskina AV, Agapova YK, Nikolaeva AY, Piatkevich KD, Patrushev MV, Boyko KM, Subach FV Biochem Biophys Res Commun. 2023 Jul 12;665:169-177. doi: , 10.1016/j.bbrc.2023.04.108. Epub 2023 Apr 28. PMID:37163937 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Proteopedia Page Contributors and Editors (what is this?)

OCA