8ove

CRYO-EM STRUCTURE OF TRYPANOSOMA BRUCEI PROCYCLIC FORM 80S RIBOSOME : TB11CS6H1 snoRNA mutant

spinqualitylabelsall models 8ove, resolution 2.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8ove is a 10 chain structure with sequence from Trypanosoma brucei brucei. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 2.6Å Ligands: 1MA, 5MC, 7MG, A2M, B8N, K, MA6, MG, NA, OMC, OMG, OMU, PSU Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q383F5_TRYB2 Publication Abstract from PubMed Trypanosomes are protozoan parasites that cycle between insect and mammalian hosts and are the causative agent of sleeping sickness. Here, we describe the changes of pseudouridine (Psi) modification on rRNA in the two life stages of the parasite using four different genome-wide approaches. CRISPR-Cas9 knock-outs of all four snoRNAs guiding Psi on helix 69 (H69) of the large rRNA subunit were lethal. A single knock-out of a snoRNA guiding Psi530 on H69 altered the composition of the 80S monosome. These changes specifically affected the translation of only a subset of proteins. This study correlates a single site Psi modification with changes in ribosomal protein stoichiometry, supported by a high-resolution cryo-EM structure. We propose that alteration in rRNA modifications could generate ribosomes preferentially translating state-beneficial proteins. A single pseudouridine on rRNA regulates ribosome structure and function in the mammalian parasite Trypanosoma brucei.,Rajan KS, Madmoni H, Bashan A, Taoka M, Aryal S, Nobe Y, Doniger T, Galili Kostin B, Blumberg A, Cohen-Chalamish S, Schwartz S, Rivalta A, Zimmerman E, Unger R, Isobe T, Yonath A, Michaeli S Nat Commun. 2023 Nov 20;14(1):7462. doi: 10.1038/s41467-023-43263-6. PMID:37985661 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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