8pd9

cAMP-bound SpSLC9C1 in lipid nanodiscs, protomer state 1

spinqualitylabelsall models 8pd9, resolution 3.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8pd9 is a 1 chain structure with sequence from Strongylocentrotus purpuratus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.6Å Ligands: CMP Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A3RL54_STRPU Publication Abstract from PubMed The newly characterized sperm-specific Na(+)/H(+) exchanger stands out by its unique tripartite domain composition(1,2). It unites a classical solute carrier unit with regulatory domains usually found in ion channels, namely, a voltage-sensing domain and a cyclic-nucleotide binding domain(1,3), which makes it a mechanistic chimera and a secondary-active transporter activated strictly by membrane voltage. Our structures of the sea urchin SpSLC9C1 in the absence and presence of ligands reveal the overall domain arrangement and new structural coupling elements. They allow us to propose a gating model, where movements in the voltage sensor indirectly cause the release of the exchanging unit from a locked state through long-distance allosteric effects transmitted by the newly characterized coupling helices. We further propose that modulation by its ligand cyclic AMP occurs by means of disruption of the cytosolic dimer interface, which lowers the energy barrier for S4 movements in the voltage-sensing domain. As SLC9C1 members have been shown to be essential for male fertility, including in mammals(2,4,5), our structure represents a potential new platform for the development of new on-demand contraceptives. Structures of a sperm-specific solute carrier gated by voltage and cAMP.,Kalienkova V, Peter MF, Rheinberger J, Paulino C Nature. 2023 Nov;623(7985):202-209. doi: 10.1038/s41586-023-06629-w. Epub 2023 , Oct 25. PMID:37880361 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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