8qwq

Magic-angle spinning NMR Structure of Opa60 in Lipid Bilayers

spinqualitylabelsall models 8qwq Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8qwq is a 1 chain structure with sequence from Neisseria gonorrhoeae. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Solid-state NMR Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function OPAH_NEIGO Implicated in a number of adherence functions. OPA proteins are implicated in pathogenesis and are subject to phase variation. Publication Abstract from PubMed Here we report the structure of Opa60 in lipid bilayers using proton-detected magic-angle spinning nuclear magnetic resonance (MAS NMR). Preparations including near-native oligosaccharide lipids reveal a consistent picture of a stable transmembrane beta barrel with a minor increase in the structured region as compared with the previously reported detergent structure. The large variable loops known to interact with host proteins could not be detected, confirming their dynamic nature even in a lipid bilayer environment. The structure provides a starting point for investigation of the functional role of Opa60 in gonococcal infection, which is understood to involve interaction with host proteins. At the same time, it demonstrates the recent advances in proton-detected methodology for membrane protein structure determination at atomic resolution by MAS NMR. Magic-angle spinning NMR structure of Opa60 in lipid bilayers.,Forster MC, Tekwani Movellan K, Najbauer EE, Becker S, Andreas LB J Struct Biol X. 2024 Feb 21;9:100098. doi: 10.1016/j.yjsbx.2024.100098. , eCollection 2024 Jun. PMID:39010882 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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