8r8c

Structure of the N-terminal domain of CMA from Cucumis melo in complex with N-acetylgalactosamine

spinqualitylabelsall models 8r8c, resolution 1.55Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8r8c is a 1 chain structure with sequence from Cucumis melo. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.55Å Ligands: A2G, CD, NGA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A1S4E5V9_CUCME Publication Abstract from PubMed Plant lectins have garnered attention for their roles as laboratory probes and potential therapeutics. Here, we report the discovery and characterization of Cucumis melo agglutinin (CMA1), a new R-type lectin from melon. Our findings reveal CMA1's unique glycan-binding profile, mechanistically explained by its 3D structure, augmenting our understanding of R-type lectins. We expressed CMA1 recombinantly and assessed its binding specificity using multiple glycan arrays, covering 1,046 unique sequences. This resulted in a complex binding profile, strongly preferring C2-substituted, beta-linked galactose (both GalNAc and Fuca1-2Gal), which we contrasted with the established R-type lectin Ricinus communis agglutinin 1 (RCA1). We also report binding of specific glycosaminoglycan subtypes and a general enhancement of binding by sulfation. Further validation using agglutination, thermal shift assays, and surface plasmon resonance confirmed and quantified this binding specificity in solution. Finally, we solved the high-resolution structure of the CMA1 N-terminal domain using X-ray crystallography, supporting our functional findings at the molecular level. Our study provides a comprehensive understanding of CMA1, laying the groundwork for further exploration of its biological and therapeutic potential. Elucidating the glycan-binding specificity and structure of Cucumis melo agglutinin, a new R-type lectin.,Lundstrom J, Gillon E, Chazalet V, Kerekes N, Di Maio A, Feizi T, Liu Y, Varrot A, Bojar D Beilstein J Org Chem. 2024 Feb 19;20:306-320. doi: 10.3762/bjoc.20.31. , eCollection 2024. PMID:38410776 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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