8rwj

cryoEM structure of Acs1 filament determined by FilamentID

spinqualitylabelsall models 8rwj, resolution 3.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8rwj is a 9 chain structure with sequence from Saccharomyces cerevisiae SK1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.5Å Ligands: 6R9 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function N1P7N2_YEASC Publication Abstract from PubMed Gamete formation and subsequent offspring development often involve extended phases of suspended cellular development or even dormancy. How cells adapt to recover and resume growth remains poorly understood. Here, we visualized budding yeast cells undergoing meiosis by cryo-electron tomography (cryoET) and discovered elaborate filamentous assemblies decorating the nucleus, cytoplasm, and mitochondria. To determine filament composition, we developed a "filament identification" (FilamentID) workflow that combines multiscale cryoET/cryo-electron microscopy (cryoEM) analyses of partially lysed cells or organelles. FilamentID identified the mitochondrial filaments as being composed of the conserved aldehyde dehydrogenase Ald4(ALDH2) and the nucleoplasmic/cytoplasmic filaments as consisting of acetyl-coenzyme A (CoA) synthetase Acs1(ACSS2). Structural characterization further revealed the mechanism underlying polymerization and enabled us to genetically perturb filament formation. Acs1 polymerization facilitates the recovery of chronologically aged spores and, more generally, the cell cycle re-entry of starved cells. FilamentID is broadly applicable to characterize filaments of unknown identity in diverse cellular contexts. FilamentID reveals the composition and function of metabolic enzyme polymers during gametogenesis.,Hugener J, Xu J, Wettstein R, Ioannidi L, Velikov D, Wollweber F, Henggeler A, Matos J, Pilhofer M Cell. 2024 Jun 20;187(13):3303-3318.e18. doi: 10.1016/j.cell.2024.04.026. PMID:38906101 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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