8thm

Beta carbonic anhydrase from the carboxysome of Cyanobium PCC 7001

spinqualitylabelsall models 8thm, resolution 2.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8thm is a 6 chain structure with sequence from Cyanobium sp. PCC 7001. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.3Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function B5ILN4_9CYAN Publication Abstract from PubMed Cyanobacterial CO(2) concentrating mechanisms (CCMs) sequester a globally consequential proportion of carbon into the biosphere. Proteinaceous microcompartments, called carboxysomes, play a critical role in CCM function, housing two enzymes to enhance CO(2) fixation: carbonic anhydrase (CA) and Rubisco. Despite its importance, our current understanding of the carboxysomal CAs found in alpha-cyanobacteria, CsoSCA, remains limited, particularly regarding the regulation of its activity. Here, we present a structural and biochemical study of CsoSCA from the cyanobacterium Cyanobium sp. PCC7001. Our results show that the Cyanobium CsoSCA is allosterically activated by the Rubisco substrate ribulose-1,5-bisphosphate and forms a hexameric trimer of dimers. Comprehensive phylogenetic and mutational analyses are consistent with this regulation appearing exclusively in cyanobacterial alpha-carboxysome CAs. These findings clarify the biologically relevant oligomeric state of alpha-carboxysomal CAs and advance our understanding of the regulation of photosynthesis in this globally dominant lineage. Cyanobacterial alpha-carboxysome carbonic anhydrase is allosterically regulated by the Rubisco substrate RuBP.,Pulsford SB, Outram MA, Forster B, Rhodes T, Williams SJ, Badger MR, Price GD, Jackson CJ, Long BM Sci Adv. 2024 May 10;10(19):eadk7283. doi: 10.1126/sciadv.adk7283. Epub 2024 May , 10. PMID:38728392 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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