8u22

A Highly Stable Variant of Corynactis Californica Green Fluorescent Protein, ccGFP 7

spinqualitylabelsall models 8u22, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8u22 is a 1 chain structure with sequence from Corynactis californica. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.8Å Ligands: C12 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q1ALD4_CORYC Publication Abstract from PubMed Fluorescent proteins (FPs) are versatile biomarkers that facilitate effective detection and tracking of macromolecules of interest in real time. Engineered FPs such as superfolder green fluorescent protein (sfGFP) and superfolder Cherry (sfCherry) have exceptional refolding capability capable of delivering fluorescent readout in harsh environments where most proteins lose their native functions. Our recent work on the development of a split FP from a species of strawberry anemone, Corynactis californica, delivered pairs of fragments with up to threefold faster complementation than split GFP. We present the biophysical, biochemical, and structural characteristics of five full-length variants derived from these split C. californica GFP (ccGFP). These ccGFP variants are more tolerant under chemical denaturation with up to 8 kcal/mol lower unfolding free energy than that of the sfGFP. It is likely that some of these ccGFP variants could be suitable as biomarkers under more adverse environments where sfGFP fails to survive. A structural analysis suggests explanations of the variations in stabilities among the ccGFP variants. Engineering highly stable variants of Corynactis californica green fluorescent proteins.,Hung LW, Terwilliger TC, Waldo GS, Nguyen HB Protein Sci. 2024 Feb;33(2):e4886. doi: 10.1002/pro.4886. PMID:38151801 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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