8u8r

Y229F/V290N/S292F Streptomyces coelicolor Laccase

spinqualitylabelsall models 8u8r, resolution 2.86Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8u8r is a 3 chain structure with sequence from Streptomyces coelicolor. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.86Å Ligands: BO3, CU, GLY, OH, PEG, PG4, PGE Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q9XAL8_STRCO Publication Abstract from PubMed The key to type 1 copper (T1Cu) function lies in the fine tuning of the CuII/I reduction potential (E degrees T1Cu) to match its redox partners, enabling efficient electron transfer in a wide range of biological systems. While the secondary coordination sphere (SCS) effects have been used to tune E degrees T1Cu in azurin over a wide range, these principles are yet to be generalized to other T1Cu-containing proteins to tune catalytic properties. To this end, we have examined the effects of Y229F, V290N and S292F mutations around the T1Cu of small laccase (SLAC) from Streptomyces coelicolor to match the E degrees T1Cu to high reduction potential fungal laccases. Using ultraviolet-visible absorption and electron paramagnetic resonance spectroscopies, together with X-ray crystallography and spectroelectrochemistry, we have probed the influence of SCS mutations on the T1Cu and corresponding E degrees T1Cu. While minimal and small increases are observed in Y229F and S292F-SLAC, the V290N mutant exhibits a major increase in E degrees T1Cu. Moreover, the influence of these mutations on E degrees T1Cu is additive, culminating in a triple mutant Y229F/V290N/S292F-SLAC with the highest E degrees T1Cu of 556 mV vs. SHE reported to date. Further activity assays indicate that all mutants retain oxygen reduction reaction activity, and display improved catalytic efficiencies (kcat/KM) relative to WT-SLAC. Increasing Reduction Potentials of Type 1 Copper Center and Catalytic Efficiency of Small Laccase from Streptomyces coelicolor through Secondary Coordination Sphere Mutations.,Wang JX, Vilbert AC, Cui C, Mirts EN, Williams LH, Kim W, Zhang YJ, Lu Y Angew Chem Int Ed Engl. 2023 Nov 5:e202314019. doi: 10.1002/anie.202314019. PMID:37926680 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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