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Structure of the human INTS9-INTS11-BRAT1 complex
Structural highlights
DiseaseBRAT1_HUMAN The disease is caused by mutations affecting the gene represented in this entry. FunctionBRAT1_HUMAN Required for activation of ATM following ionizing radiation. May act by regulating dephosphorylation of ATM.[1] Publication Abstract from PubMedINTS11 and CPSF73 are metal-dependent endonucleases for Integrator and pre-mRNA 3'-end processing, respectively. Here, we show that the INTS11 binding partner BRAT1/CG7044, a factor important for neuronal fitness, stabilizes INTS11 in the cytoplasm and is required for Integrator function in the nucleus. Loss of BRAT1 in neural organoids leads to transcriptomic disruption and precocious expression of neurogenesis-driving transcription factors. The structures of the human INTS9-INTS11-BRAT1 and Drosophila dIntS11-CG7044 complexes reveal that the conserved C terminus of BRAT1/CG7044 is captured in the active site of INTS11, with a cysteine residue directly coordinating the metal ions. Inspired by these observations, we find that UBE3D is a binding partner for CPSF73, and UBE3D likely also uses a conserved cysteine residue to directly coordinate the active site metal ions. Our studies have revealed binding partners for INTS11 and CPSF73 that behave like cytoplasmic chaperones with a conserved impact on the nuclear functions of these enzymes. , PMID:39032490[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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