Structural highlights
8urp is a 2 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Method: | Electron Microscopy, Resolution 3.6Å |
| Ligands: | ACE, CDC, MG, PCF, PCW |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
CPT1_YEAST Catalyzes the final step in the CDP-choline route leading to phosphatidylcholin (PC). Preferentially uses CDP-monomethylethanolamine as aminoalcohol substrate. Shows highest activity toward di- and mono-unsaturated diacylglycerol species as lipid substrates. The CDP-choline pathway only contributes to net PC synthesis if exogenous choline is present. In its absence, this pathway recycles choline from PC turnover and may contribute to maintaining the proper PC species composition.[1] [2] [3] [4] [5]
References
- ↑ Boumann HA, de Kruijff B, Heck AJ, de Kroon AI. The selective utilization of substrates in vivo by the phosphatidylethanolamine and phosphatidylcholine biosynthetic enzymes Ept1p and Cpt1p in yeast. FEBS Lett. 2004 Jul 2;569(1-3):173-7. PMID:15225629 doi:10.1016/j.febslet.2004.05.043
- ↑ Hjelmstad RH, Bell RM. sn-1,2-diacylglycerol choline Saccharomyces cerevisiae. Mixed micellar analysis of the CPT1 and EPT1 gene products. J Biol Chem. 1991 Mar 5;266(7):4357-65 PMID:1847919
- ↑ Hjelmstad RH, Bell RM. Mutants of Saccharomyces cerevisiae defective in sn-1,2-diacylglycerol cholinephosphotransferase. Isolation, characterization, and cloning of the CPT1 gene. J Biol Chem. 1987 Mar 15;262(8):3909-17 PMID:3029130
- ↑ McGee TP, Skinner HB, Bankaitis VA. Functional redundancy of CDP-ethanolamine and CDP-choline pathway enzymes in phospholipid biosynthesis: ethanolamine-dependent effects on steady-state membrane phospholipid composition in Saccharomyces cerevisiae. J Bacteriol. 1994 Nov;176(22):6861-8. PMID:7961445 doi:10.1128/jb.176.22.6861-6868.1994
- ↑ McMaster CR, Bell RM. Phosphatidylcholine biosynthesis in Saccharomyces cerevisiae. Regulatory insights J Biol Chem. 1994 Nov 11;269(45):28010-6 PMID:7961735
