8vxq

Cryo-EM structure of phage DEV ejection proteins gp72:gp73

spinqualitylabelsall models 8vxq, resolution 3.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8vxq is a 18 chain structure with sequence from Pseudomonas phage vB_PaeP_DEV. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.1Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed DEV is an obligatory lytic Pseudomonas phage of the N4-like genus, recently reclassified as Schitoviridae. The DEV genome encodes 91 ORFs, including a 3398 amino acid virion-associated RNA polymerase (vRNAP). Here, we describe the complete architecture of DEV, determined using a combination of cryo-electron microscopy localized reconstruction, biochemical methods, and genetic knockouts. We built de novo structures of all capsid factors and tail components involved in host attachment. We demonstrate that DEV long tail fibers are essential for infection of Pseudomonas aeruginosa but dispensable for infecting mutants with a truncated lipopolysaccharide devoid of the O-antigen. We determine that DEV vRNAP is part of a three-gene operon conserved in 191 Schitoviridae genomes. We propose these three proteins are ejected into the host to form a genome ejection motor spanning the cell envelope. We posit that the design principles of the DEV ejection apparatus are conserved in all Schitoviridae. Integrative structural analysis of Pseudomonas phage DEV reveals a genome ejection motor.,Lokareddy RK, Hou CD, Forti F, Iglesias SM, Li F, Pavlenok M, Horner DS, Niederweis M, Briani F, Cingolani G Nat Commun. 2024 Oct 1;15(1):8482. doi: 10.1038/s41467-024-52752-1. PMID:39353939 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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