8w19

Cryo-EM structure of BTV star-subcore

spinqualitylabelsall models 8w19, resolution 4.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8w19 is a 15 chain structure with sequence from Bluetongue virus (serotype 1 / isolate South Africa). Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 4.4Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q1AE73_9REOV Publication Abstract from PubMed Unlike those of double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), and ssRNA viruses, the mechanism of genome packaging of dsRNA viruses is poorly understood. Here, we combined the techniques of high-resolution cryoelectron microscopy (cryo-EM), cellular cryoelectron tomography (cryo-ET), and structure-guided mutagenesis to investigate genome packaging and capsid assembly of bluetongue virus (BTV), a member of the Reoviridae family of dsRNA viruses. A total of eleven assembly states of BTV capsid were captured, with resolutions up to 2.8 A, with most visualized in the host cytoplasm. ATPase VP6 was found underneath the vertices of capsid shell protein VP3 as an RNA-harboring pentamer, facilitating RNA packaging. RNA packaging expands the VP3 shell, which then engages middle- and outer-layer proteins to generate infectious virions. These revealed "duality" characteristics of the BTV assembly mechanism reconcile previous contradictory co-assembly and core-filling models and provide insights into the mysterious RNA packaging and capsid assembly of Reoviridae members and beyond. RNA genome packaging and capsid assembly of bluetongue virus visualized in host cells.,Xia X, Sung PY, Martynowycz MW, Gonen T, Roy P, Zhou ZH Cell. 2024 Apr 25;187(9):2236-2249.e17. doi: 10.1016/j.cell.2024.03.007. Epub , 2024 Apr 12. PMID:38614100 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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