8xf2

Crystal structure of the reassembled C-phycocyanin hexamer from Thermoleptolyngbya sp. O-77

spinqualitylabelsall models 8xf2, resolution 1.98Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8xf2 is a 12 chain structure with sequence from Leptolyngbya sp. O-77. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.98Å Ligands: CYC, MEN, NA, PO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed C-phycocyanin (CPC), which contains open-chain tetrapyrroles, is a major light-harvesting red-fluorescent protein with an important role in aquatic photosynthesis. Recently, we reported a non-conventional CPC from Thermoleptolyngbya sp. O-77 (CPC(O77)) that contains two different structures, i.e., a hexameric structure and a non-conventional octameric structure. However, the assembly and disassembly mechanisms of the non-conventional octameric form of CPC remain unclear. To understand this assembly mechanism, we performed an in vitro experiment to study the disassembly and reassembly behaviors of CPC using isolated CPC subunits. The dissociation of the CPC(O77) subunit was performed using a Phenyl-Sepharose column in 20 mM potassium phosphate buffer (pH 6.0) containing 7.0 M urea. For the first time, crystals of isolated CPC subunits were obtained and analyzed after separation. After the removal of urea from the purified alpha and beta subunits, we performed an in vitro reassembly experiment for CPC and analyzed the reconstructed CPC using spectrophotometric and X-ray crystal structure analyses. The crystal structure of the reassembled CPC was nearly identical to that of the original CPC(O77). The findings of this study indicate that the octameric CPC(O77) is a naturally occurring form in the thermophilic cyanobacterium Thermoleptolyngbya sp. O-77. Disassembly and reassembly of the non-conventional thermophilic C-phycocyanin.,Nguyen HK, Minato T, Teramoto T, Ogo S, Kakuta Y, Yoon KS J Biosci Bioeng. 2024 Mar;137(3):179-186. doi: 10.1016/j.jbiosc.2023.12.015. Epub , 2024 Jan 18. PMID:38238241 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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