8y6v

Near-atomic structure of icosahedrally averaged jumbo bacteriophage PhiKZ capsid

spinqualitylabelsall models 8y6v, resolution 3.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8y6v is a 41 chain structure with sequence from Pseudomonas virus phiKZ. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.5Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Jumbo phages are a group of tailed bacteriophages with large genomes and capsids. As a prototype of jumbo phage, PhiKZ infects Pseudomonas aeruginosa, a multi-drug-resistant (MDR) opportunistic pathogen leading to acute or chronic infection in immunocompromised individuals. It holds potential to be used as an antimicrobial agent and as a model for uncovering basic phage biology. Although previous low-resolution structural studies have indicated that jumbo phages may have more complicated capsid structures than smaller phages such as HK97, the detailed structures and the assembly mechanism of their capsids remain largely unknown. Here, we report a 3.5-A-resolution cryo-EM structure of the PhiKZ capsid. The structure unveiled ten minor capsid proteins, with some decorating the outer surface of the capsid and the others forming a complex network attached to the capsid's inner surface. This network seems to play roles in driving capsid assembly and capsid stabilization. Similar mechanisms of capsid assembly and stabilization are probably employed by many other jumbo viruses. Capsid structure of bacteriophage PhiKZ provides insights into assembly and stabilization of jumbo phages.,Yang Y, Shao Q, Guo M, Han L, Zhao X, Wang A, Li X, Wang B, Pan JA, Chen Z, Fokine A, Sun L, Fang Q Nat Commun. 2024 Aug 2;15(1):6551. doi: 10.1038/s41467-024-50811-1. PMID:39095371 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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