8y9q

b-glucosidase from Thermotoga profunda Tp-BGL

spinqualitylabelsall models 8y9q, resolution 1.96Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8y9q is a 2 chain structure with sequence from Thermotoga profunda. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.96Å Ligands: GOL Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed beta-Glucosidase is a crucial cellulase, as its activity determines the efficiency of cellulose hydrolysis into glucose. This study addresses the functional and structural characteristics of Thermotoga profunda beta-glucosidase (Tp-BGL). Tp-BGL exhibited a K(m) of 0.3798 mM for p-nitrophenyl-beta-d-glucopyranoside (pNPGlc) and 4.44 mM for cellobiose, with k(cat)/K(m) of 1211.16 and 4.18 s(-1) mM(-1), respectively. In addition, Tp-BGL showed significant pH adaptability and thermal stability, with a T(m) of 85.7 degrees C and retaining >90 % of its activity after incubation at 80 degrees C for 90 min. The crystal structure of Tp-BGL was resolved at 1.95 A resolution, and reveals a typical TIM barrel structure. Comparative structural analysis highlighted that the major distinction between Tp-BGL and the other glucosidases lies in their loop regions. Structural insights and functional characterization of a novel beta-glucosidase derived from Thermotoga profunda.,Guo Y, Chen A, Liu K, Ji C Biochem Biophys Res Commun. 2024 Nov 5;732:150405. doi: , 10.1016/j.bbrc.2024.150405. Epub 2024 Jul 14. PMID:39033552 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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