8yqh

Crystal structure of Cry3Aa-PEI at 3.1 A

spinqualitylabelsall models 8yqh, resolution 3.11Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8yqh is a 1 chain structure with sequence from Bacillus thuringiensis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.11Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q9S6N9_BACTU Publication Abstract from PubMed NAD(H)-dependent enzymes play a crucial role in the biosynthesis of pharmaceuticals and fine chemicals, but the limited recyclability of the NAD(H) cofactor hinders its more general application. Here, we report the generation of mechano-responsive PEI-modified Cry3Aa protein crystals and their use for NADH recycling over multiple reaction cycles. For demonstration of its practical utility, a complementary Cry3Aa protein particle containing genetically encoded and co-immobilized formate dehydrogenase for NADH regeneration and leucine dehydrogenase for catalyzing the NADH-dependent l-tert-leucine (l-tert-Leu) biosynthesis has been produced. When combined with the PEI-modified Cry3Aa crystal, the resultant reaction system could be used for the efficient biosynthesis of l-tert-Leu for up to 21 days with a 10.5-fold improvement in the NADH turnover number. Mechanoresponsive Protein Crystals for NADH Recycling in Multicycle Enzyme Reactions.,Yekta R, Xiong X, Li J, Heater BS, Lee MM, Chan MK J Am Chem Soc. 2024 Jul 17;146(28):18817-18822. doi: 10.1021/jacs.4c04725. Epub , 2024 Jul 5. PMID:38968608 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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