8yv9

Crystal structure of Caenorhabditis elegans HIM-8 ZF1-2-CTD domain in complex with Chromosome X pairing center

spinqualitylabelsall models 8yv9, resolution 2.14Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8yv9 is a 3 chain structure with sequence from Caenorhabditis elegans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.14Å Ligands: ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function O18068_CAEEL Publication Abstract from PubMed Pairing center (PC) on each chromosome of Caenorhabditis elegans is crucial for homolog pairing and initiating synapsis. Within each PC, clusters of 11/12 bp DNA motif recruit one of four paralogous meiosis-specific proteins: ZIM-1, ZIM-2, ZIM-3, or HIM-8. However, the mechanistic basis underlying the specificity of ZIM/HIM-8-PC DNA interaction remains elusive. Here, we describe crystal structures of HIM-8, ZIM-1 and ZIM-2 DNA binding domains (ZF1, ZF2 and CTD) in complex with their cognate PC DNA motifs, respectively. These structures demonstrated the ZF1-2-CTD folds as an integrated structural unit crucial for its DNA binding specificity. Base-specific DNA-contacting residues are exclusively distributed on ZF1-2 and highly conserved. Furthermore, the CTD potentially contributes to the conformational diversity of ZF1-2, imparting binding specificity to distinct PC DNA motifs. These findings shed light on the mechanism governing PC DNA motif recognition by ZIM/HIM-8 proteins, suggesting a co-evolution relationship between PC DNA motifs and ZF1-2-CTD in shaping the specific recognition. Structural basis for C. elegans pairing center DNA binding specificity by the ZIM/HIM-8 family proteins.,Li M, Zhu C, Xu Z, Xu M, Kuang Y, Hou X, Huang X, Lv M, Liu Y, Zhang Y, Xu Z, Han X, Wang S, Shi Y, Guang S, Li F Nat Commun. 2024 Nov 28;15(1):10355. doi: 10.1038/s41467-024-54548-9. PMID:39609407 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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