8zr9

Cryo-EM structure of AbCapV filemant bound with 3',3'-cGAMP

spinqualitylabelsall models 8zr9, resolution 2.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8zr9 is a 6 chain structure with sequence from Acinetobacter baumannii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 2.7Å Ligands: 4BW Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A505MGQ1_ACIBA Publication Abstract from PubMed Cyclic oligonucleotide-based antiphage signaling systems (CBASS) are widespread bacterial immune systems that trigger host suicide via cyclic nucleotide-activated effectors. The predominant strategy to induce cell death in CBASS is membrane disruption. Here, we demonstrate that patatin-like phospholipase CapV, the most abundant CBASS effector, relocates and cleaves membrane phospholipids at the cell pole upon 3'3'-cGAMP binding, inducing polarized membrane disruption and cell death. Using cryo-EM, we reveal that apo-CapV adopts both dimeric and tetrameric states, with its phospholipid-binding pocket occluded and locked in an inactive conformation. Binding to 3'3'-cGAMP induces filamentation and substantial conformational change of CapV, enhancing membrane binding via electrostatic interactions between its interspaced basic surfaces and the negatively charged phosphate moieties of phospholipids. Simultaneously, the rearrangement opens the phospholipid-binding pocket, enabling the accommodation of two fatty acid chains of phospholipid within distinct hydrophobic pockets. Our findings reveal a filament-dependent activation mechanism for phospholipase-mediated membrane disruption during antiviral response. Molecular mechanisms of CBASS phospholipase effector CapV mediated membrane disruption.,Kong J, Wu W, Ke S, Zhou Z, Xia S, Chen J, Zhu R, Hou Y, Makanyire T, Shan X, Zhuo Z, Li K, Shen H, Yang P, Huang P, Liu J, Li J, Sun X, Dong J, Sun H, Chen M, Lu M, Li Z, Xiao Y Nat Commun. 2025 Sep 29;16(1):8611. doi: 10.1038/s41467-025-63658-x. PMID:41022836 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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