8zvg

Crystal structure of AetD in complex with L-tyrosine

spinqualitylabelsall models 8zvg, resolution 1.89Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8zvg is a 2 chain structure with sequence from Aetokthonos hydrillicola Thurmond2011. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.89Å Ligands: FE2, NI, TYR Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A861B387_9CYAN Publication Abstract from PubMed Organohalogen compounds exhibit wide-ranging bioactivities and potential applications. Understanding natural biosynthetic pathways and improving the production of halogenated compounds has garnered significant attention. Recently, the biosynthetic pathway of a cyanobacterial neurotoxin, aetokthonotoxin, was reported. It contains two unique enzymes: a single-component flavin-dependent halogenase AetF and a new type of nitril synthase AetD. The crystal structures of these enzymes in complex with their cofactors and substrates that were recently reported will be presented here. The AetF structures reveal a tri-domain architecture, the transfer direction of the hydride ion, a possible path to deliver the hypohalous acid, and the unusual bispecific substrate-recognition mode. The AetD structures demonstrate that the nitrile formation should occur through the action of a diiron cluster, implying that the enzyme should be capable of catalyzing the nitrile formation of alternative amino acids. This information is of central importance for understanding the mechanism of action as well as the applications of these two the-first-of-its-kind enzymes. Structural and molecular insights of two unique enzymes involved in the biosynthesis of a natural halogenated nitrile.,Chen CC, Li H, Huang JW, Guo RT FEBS J. 2024 Sep 22. doi: 10.1111/febs.17279. PMID:39308083 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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