8zym

Complex structure of 60 Fab bound to DS2 prefusion F trimer

spinqualitylabelsall models 8zym, resolution 3.23Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8zym is a 9 chain structure with sequence from Mus musculus, Respiratory syncytial virus A2 and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.23Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function C3UPB8_9MONO Publication Abstract from PubMed DS-Cav1, SC-TM, and DS2 are distinct designer pre-fusion F proteins (pre-F) of respiratory syncytial virus (RSV) developed for vaccines. However, their immunogenicity has not been directly compared. In this study, we generated three recombinant vaccines using the chimpanzee adenovirus vector AdC68 to express DS-Cav1, SC-TM, and DS2. All three vaccines elicited robust serum binding and neutralizing antibodies following intramuscular priming and boosting. DS2 induced the strongest antibody responses, followed by SC-TM and DS-Cav1. DS2 also provided strong protection against live RSV challenge. Monoclonal antibodies (mAbs) isolated from long-lived antibody-secreting cells (ASCs) in the bone marrow six months post-immunization with AdC68-DS2 predominantly targeted site O as well as site II. One neutralizing antibody against site II, mAb60, conferred strong protection against live RSV infection in mice. These findings highlight the strong ability of the DS2 design in eliciting long-lived antibody responses and guide the development of next-generation RSV vaccines. DS2 designer pre-fusion F vaccine induces strong and protective antibody response against RSV infection.,Yang Y, Wang R, Guo F, Zhao T, Lei Y, Yang Q, Zeng Y, Yang Z, Ajavavarakula T, Tan R, Li M, Dong H, Niu M, Bao K, Geng H, Lv Q, Zhang Q, Shi X, Liu P, Ge J, Wang X, Zhang L NPJ Vaccines. 2024 Dec 31;9(1):258. doi: 10.1038/s41541-024-01059-9. PMID:39741146 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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