9b42

Pseudomonas phage Pa193 neck and extended tail (collar, gateway, tail tube, and sheath proteins)

spinqualitylabelsall models 9b42, resolution 3.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9b42 is a 19 chain structure with sequence from Pseudomonas virus Pa193. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.5Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A5P1KVA0_9CAUD Publication Abstract from PubMed The World Health Organization has designated Pseudomonas aeruginosa as a critical pathogen for the development of new antimicrobials. Bacterial viruses, or bacteriophages, have been used in various clinical settings, commonly called phage therapy, to address this growing public health crisis. Here, we describe a high-resolution structural atlas of a therapeutic, contractile-tailed Pseudomonas phage, Pa193. We used bioinformatics, proteomics, and cryogenic electron microscopy single particle analysis to identify, annotate, and build atomic models for 21 distinct structural polypeptide chains forming the icosahedral capsid, neck, contractile tail, and baseplate. We identified a putative scaffolding protein stabilizing the interior of the capsid 5-fold vertex. We also visualized a large portion of Pa193 ~ 500 A long tail fibers and resolved the interface between the baseplate and tail fibers. The work presented here provides a framework to support a better understanding of phages as biomedicines for phage therapy and inform engineering opportunities. Cryo-EM analysis of Pseudomonas phage Pa193 structural components.,Iglesias SM, Hou CD, Reid J, Schauer E, Geier R, Soriaga A, Sim L, Gao L, Whitelegge J, Kyme P, Birx D, Lemire S, Cingolani G Commun Biol. 2024 Oct 6;7(1):1275. doi: 10.1038/s42003-024-06985-x. PMID:39370451 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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