9bf5

Structure of V. cholerae DdmD in complex with ssDNA

spinqualitylabelsall models 9bf5, resolution 3.07Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9bf5 is a 4 chain structure with sequence from Vibrio cholerae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.07Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function B9TSM3_VIBCL Publication Abstract from PubMed Horizontal gene transfer is a key driver of bacterial evolution, but it also presents severe risks to bacteria by introducing invasive mobile genetic elements. To counter these threats, bacteria have developed various defense systems, including prokaryotic Argonautes (pAgos) and the DNA defense module DdmDE system. Through biochemical analysis, structural determination, and in vivo plasmid clearance assays, we elucidate the assembly and activation mechanisms of DdmDE, which eliminates small, multicopy plasmids. We demonstrate that DdmE, a pAgo-like protein, acts as a catalytically inactive, DNA-guided, DNA-targeting defense module. In the presence of guide DNA, DdmE targets plasmids and recruits a dimeric DdmD, which contains nuclease and helicase domains. Upon binding to DNA substrates, DdmD transitions from an autoinhibited dimer to an active monomer, which then translocates along and cleaves the plasmids. Together, our findings reveal the intricate mechanisms underlying DdmDE-mediated plasmid clearance, offering fundamental insights into bacterial defense systems against plasmid invasions. DdmDE eliminates plasmid invasion by DNA-guided DNA targeting.,Yang XY, Shen Z, Wang C, Nakanishi K, Fu TM Cell. 2024 Aug 13:S0092-8674(24)00822-5. doi: 10.1016/j.cell.2024.07.028. PMID:39173632 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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