9c5d

E. coli peptidyl-prolyl cis-trans isomerase containing (2S,3S)-4-Fluorovaline

spinqualitylabelsall models 9c5d, resolution 1.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9c5d is a 4 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.3Å Ligands: 1PE, A1AVU, ACT, EDO, PEG, PGE, XPE Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function PPIB_ECOLI PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Publication Abstract from PubMed (2S,3S)-4-Fluorovaline (FVal) is an analogue of valine, where a single CH(3) group is substituted by a CH(2)F group. In the absence of valine, E. coli valyl-tRNA synthetase uses FVal as a substitute, enabling the production of proteins uniformly labeled with FVal. Here, we describe the production and analysis of E. coli peptidyl-prolyl isomerase B where all 16 valine residues have been replaced by FVal synthesized with a (13)C-labeled CH(2)F group. Although the melting temperature is lower by about 11 degrees C relative to the wild-type protein, the three-dimensional protein structure is almost completely conserved, as shown by X-ray crystallography. The CH(2)F groups invariably populate staggered rotamers. Most CH(2)F groups populate two different rotamers. The increased space requirement of fluorine versus hydrogen does not prohibit rotamers that position fluorine next to a backbone carbonyl carbon. (19)F NMR spectra show a signal dispersion over 25 ppm. The most high-field shifted (19)F resonances correlate with large (3)J(HF) coupling constants, confirming the impact of the gamma-gauche effect on the signal dispersion. The present work is the second experimental verification of the effect and extends its validity to fluorovaline. The abundance of valine in proteins and structural conservation with FVal renders this valine analogue attractive for probing proteins by (19)F NMR spectroscopy. 1.3 A Crystal Structure of E. coli Peptidyl-Prolyl Isomerase B with Uniform Substitution of Valine by (2S,3S)-4-Fluorovaline Reveals Structure Conservation and Multiple Staggered Rotamers of CH(2)F Groups.,Frkic RL, Tan YJ, Maleckis A, Chilton NF, Otting G, Jackson CJ Biochemistry. 2024 Sep 24. doi: 10.1021/acs.biochem.4c00345. PMID:39316701 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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