9cai
High-resolution C. elegans 80S ribosome structure - class 2
Structural highlights
FunctionRSSA_CAEEL Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Involved in cold-warm shock-induced translocation of the RNA exosome components from the nucleolus to nucleoplasm (PubMed:36763670).[HAMAP-Rule:MF_03015][1] Publication Abstract from PubMedCaenorhabditis elegans is an important model organism for human health and disease, with foundational contributions to the understanding of gene expression and tissue patterning in animals. An invaluable tool in modern gene expression research is the presence of a high-resolution ribosome structure, though no such structure exists for C. elegans. Here we present a high-resolution single-particle cryogenic electron microscopy (cryoEM) reconstruction and molecular model of a C. elegans ribosome, revealing a significantly streamlined animal ribosome. Many facets of ribosome structure are conserved in C. elegans, including overall ribosomal architecture and the mechanism of cycloheximide, while other facets such as expansion segments and eL28 are rapidly evolving. We identify uL5 and uL23 as two instances of tissue-specific ribosomal protein paralog expression conserved in Caenorhabditis, suggesting that C. elegans ribosomes vary across tissues. The C. elegans ribosome structure will provide a basis for future structural, biochemical, and genetic studies of translation in this important animal system. High-Resolution Reconstruction of a C. elegans Ribosome Sheds Light on Evolutionary Dynamics and Tissue Specificity.,Sehgal E, Wohlenberg C, Soukup EM, Viscardi MJ, Serrao VHB, Arribere JA RNA. 2024 Aug 29:rna.080103.124. doi: 10.1261/rna.080103.124. PMID:39209556[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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