9cqf

CRYSTAL STRUCTURE OF APO C-TERMINAL HIS-TAG DOG HSP47(36-418) IN A C 2 2 21 CRYSTAL FORM

spinqualitylabelsall models 9cqf, resolution 2.93Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9cqf is a 8 chain structure with sequence from Canis lupus familiaris. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.928Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function C7C419_CANLF Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.[ARBA:ARBA00025405] Publication Abstract from PubMed Heat-shock protein 47 (HSP47) is a potential target for inhibitors that ameliorate fibrosis by reducing collagen assembly. In an effort to develop a structure-based drug-design system, it was not possible to replicate a previous literature result (PDB entry 4au4) for apo dog HSP47; instead, crystal forms were obtained in which pairs of dog HSP47 molecules interacted through a noncleavable C-terminal His-tag to build up tetramers, all of which had multiple molecules of HSP47 in the asymmetric unit and none of which diffracted as well as the literature precedent. To overcome these difficulties, a two-pronged approach was followed: (i) the His-tag was moved from the C-terminus to the N-terminus and was made cleavable, and (ii) Adnectin (derived from the tenth domain of human fibronectin type III) crystallization chaperones were developed. Both approaches provided well diffracting crystals, but the latter approach yielded crystal forms with only one or two HSP47 complexes per asymmetric unit, which made model building less onerous. Improving the diffraction quality of heat-shock protein 47 crystals.,Kish K, Cobell S, Szapiel N, Yan C, Newitt JA, Tredup J, Rodrigo I, Tomasco E, Gao M, Marsilio F, Haugner J, Lipovsek D, Deng B, Bousquet P, Zhang Y, Schmidt H, Sheriff S Acta Crystallogr F Struct Biol Commun. 2024 Nov 1. doi: , 10.1107/S2053230X24009233. PMID:39397789 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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