9cuf

Room temperature SSX structure of ccNiR

spinqualitylabelsall models 9cuf, resolution 3.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9cuf is a 2 chain structure with sequence from Shewanella oneidensis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.3Å Ligands: CA, HEC, PE3 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function NRFA_SHEON Plays a role in nitrite reduction.[HAMAP-Rule:MF_01182] Publication Abstract from PubMed The upgrade of the European Synchrotron Radiation Facility (ESRF) in Grenoble, France to an Extremely Brilliant Source (EBS) is expected to enable time-resolved synchrotron serial crystallography (SSX) experiments with sub-millisecond time resolution. ID29 is a new beamline dedicated to SSX experiments at ESRF-EBS. Here, we report experiments emerging from the initial phase of user operation at ID29. We first used microcrystals of photoactive yellow protein as a model system to exploit the potential of microsecond pulses for SSX. Subsequently, we investigated microcrystals of cytochrome c nitrite reductase (ccNiR) with microsecond X-ray pulses. CcNiR is a decaheme protein that is ideal for the investigation of radiation damage at the various heme-iron sites. Finally, we performed a proof-of-concept subsecond time-resolved SSX experiment by photoactivating microcrystals of a myxobacterial phytochrome. Exploiting fourth-generation synchrotron radiation for enzyme and photoreceptor characterization.,Malla TN, Muniyappan S, Menendez D, Ogukwe F, Dale AN, Clayton JD, Weatherall DD, Karki P, Dangi S, Mandella V, Pacheco AA, Stojkovic EA, Rose SL, Orlans J, Basu S, de Sanctis D, Schmidt M IUCrJ. 2025 Jan 1;12(Pt 1):36-48. doi: 10.1107/S2052252524010868. PMID:39575537 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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