9cv8

Crystal Structure of Cytochrome P450 NysL bound to nystatin

spinqualitylabelsall models 9cv8, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9cv8 is a 2 chain structure with sequence from Streptomyces noursei. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2Å Ligands: A1AZ7, HEM, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q9L4X0_STRNR Publication Abstract from PubMed NysL, a cytochrome P450 monooxygenase from the Gram-positive bacterium Streptomyces noursei, catalyzes the C10 hydroxylation of 10-deoxynystain to nystatin A(1), a clinically important antifungal. In this study, we present the 2.0 A resolution crystal structure of NysL bound to nystatin A(1). The structure of this complex provides key insights into the structural elements that dictate the regio- and stereo- selective oxidation of large 20-44-membered macrolide substrates. The closely related AmphL operates on a similar 38-member macrolide but oxidizes C8 rather than C10. This difference requires that the substrate for AmphL penetrate further into the active site relative to NysL. The depth of substrate penetration is controlled by interactions between an area of the substrate binding pocket deemed the "back-wall" and the hemiketal ring of the macrolide substrate. Crystal structure of cytochrome P450 NysL and the structural basis for stereo- and regio- selective oxidation of antifungal macrolides.,Murarka VC, Kim JS, Lamb DC, Kelly SL, Poulos TL, Follmer AH J Biol Chem. 2025 Jan 13:108185. doi: 10.1016/j.jbc.2025.108185. PMID:39814230 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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