9d01

Crystal Structure of a-iTHR-201

spinqualitylabelsall models 9d01, resolution 2.18Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9d01 is a 1 chain structure with sequence from Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.18Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Given the repetitive structure of crystalline ice, it is unsurprising that highly active ice-binding proteins (IBPs), often with beta-roll structures, also have repeating motifs. Here, we introduce a de novo designed family of ice-binding twistless alpha-helical repeat (iTHR) proteins. Each iTHR protein comprises two planar layers of parallel alpha-helices connected by loops-a structural topology not seen in native IBPs. The ice-binding helices contain an ordered array of TXXXAXXXAXX motifs, precisely spaced to complement the pyramidal 201 and secondary prism 110 planes of the ice lattice, with a designed 98.2 degrees residue turn angle that orients all threonines uniformly toward the ice surface. iTHR proteins show high solubility, thermostability, and produce varied ice crystal morphologies depending on their intended target facet. Crucially, iTHRs exhibit ice recrystallization inhibition (IRI) at critical concentrations comparable to those of many native globular IBPs. Extensive site-specific mutagenesis shows that ice-binding activity in iTHR proteins is robust, remaining largely unaffected by changes in chemical composition. Variation in the repeat number reveals a nonmonotonic relationship to IRI activity. X-ray crystal structures of two designs confirm the intended orientation of threonines, uniformly pointing toward the ice surface. The iTHR family provides a versatile platform to systematically investigate the complex structure-activity relationships underlying protein-ice interactions. Inhibition of ice recrystallization with designed twistless helical repeat proteins.,de Haas RJ, Pyles H, Huddy EB, van Ossenbruggen J, Zheng C, van den Broek D, Giezen SN, Carr A, Bera AK, Kang A, Brackenbrough E, Joyce E, Sankaran B, Baker D, Voets IK, de Vries R Proc Natl Acad Sci U S A. 2025 Dec 2;122(48):e2514871122. doi: , 10.1073/pnas.2514871122. Epub 2025 Nov 25. PMID:41289379 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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