9d5n

48-nm doublet microtubule from Trichomonas vaginalis strain G3

spinqualitylabelsall models 9d5n, resolution 4.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9d5n is a 215 chain structure with sequence from Trichomonas vaginalis G3. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 4.2Å Ligands: GDP, GTP, MG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A2FZ95_TRIV3 Publication Abstract from PubMed Doublet microtubules (DMTs) are flagellar components required for the protist Trichomonas vaginalis (Tv) to swim through the human genitourinary tract to cause trichomoniasis, the most common non-viral sexually transmitted disease. Lack of structures of Tv's DMT (Tv-DMT) has prevented structure-guided drug design to manage Tv infection. Here, we determine the 16 nm, 32 nm, 48 nm and 96 nm-repeat structures of native Tv-DMT at resolution ranging from 3.4 to 4.4 A by cryogenic electron microscopy (cryoEM) and built an atomic model for the entire Tv-DMT. These structures show that Tv-DMT is composed of 30 different proteins, including the alpha- and beta-tubulin, 19 microtubule inner proteins (MIPs) and 9 microtubule outer proteins. While the A-tubule of Tv-DMT is simplistic compared to DMTs of other organisms, the B-tubule of Tv-DMT features parasite-specific proteins, such as TvFAP40 and TvFAP35. Notably, TvFAP40 and TvFAP35 form filaments near the inner and outer junctions, respectively, and interface with stabilizing MIPs. This atomic model of the Tv-DMT highlights diversity of eukaryotic motility machineries and provides a structural framework to inform rational design of therapeutics against trichomoniasis. Structures of Native Doublet Microtubules from Trichomonas vaginalis Reveal Parasite-Specific Proteins.,Stevens A, Kashyap S, Crofut EH, Wang SE, Muratore KA, Johnson PJ, Zhou ZH Nat Commun. 2025 Apr 29;16(1):3996. doi: 10.1038/s41467-025-59369-y. PMID:40301421 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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