| Structural highlights
Function
RUVB_ECOLI The RuvABC complex is involved in recombinational repair of UV or chemically damaged DNA (PubMed:6374379). The complex also plays an important role in the rescue of blocked DNA replication forks via replication fork reversal (RFR); RFR and homologous recombination required for UV light survival can be separated (PubMed:16424908, PubMed:18942176, PubMed:9814711). This subunit has a weak ATPase activity that is inhibited by its ADP product; binds ADP better than ATP (PubMed:2529252). Promotes Holliday junction (HJ) branch migration in conjunction with RuvA. Binds to HJ cruciform DNA; in the presence of RuvA, ATP and Mg(2+) the junction is dissociated. Hydrolyzable (d)NTPs can replace ATP but other analogs cannot (PubMed:1608954, PubMed:1617728, PubMed:6374379, PubMed:8393934). The RuvB hexamer acts as a pump, pulling DNA into and through the RuvAB complex (PubMed:9078376). Can bypass UV-induced lesions (PubMed:1617728) and physically cross-linked DNA strands (PubMed:10662672), suggesting RuvB does not unwind large sections of DNA. RuvA gives specificity by binding to cruciform junctions, while the RuvB ATPase provides the motor force for branch migration; excess RuvB can promote branch migration in the absence of RuvA (PubMed:10662672, PubMed:1617728). In vitro the RuvA-RuvB complex has 5'-3' helicase activity that is ATP-dependent and works best on short dsDNA hybrids; 52 and 66-nucleotide (nt) pairs are easily displaced, hybrids greater than 140-nts are not (PubMed:8433990). RuvA stimulates the weak ATPase activity of RuvB in the presence of DNA; HJ DNA stimulates ATPase about 10-fold (PubMed:1435721, PubMed:1833759, PubMed:8393934).[HAMAP-Rule:MF_00016][1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11] [12] [13] An in vitro resolvase system that forms and processes HJ has been reconstituted with DNA substrates, RuvA, RuvB and RuvC. RuvA-RuvB increases the rate of strand exchange (branch migration), dissociates the RecA filament and allows RuvC to cleave in both orientations at the cruciform junction (PubMed:10421637, PubMed:9160752). HJ-RuvA-RuvB-RuvC complexes resolve Holliday junctions and also undergo branch migration, providing evidence for a coupled branch migration/HJ resolution reaction (PubMed:10421637).[14] [15]
References
- ↑ George H, Kuraoka I, Nauman DA, Kobertz WR, Wood RD, West SC. RuvAB-mediated branch migration does not involve extensive DNA opening within the RuvB hexamer. Curr Biol. 2000 Jan 27;10(2):103-6. PMID:10662672 doi:10.1016/s0960-9822(00)00296-7
- ↑ Tsaneva IR, Illing G, Lloyd RG, West SC. Purification and properties of the RuvA and RuvB proteins of Escherichia coli. Mol Gen Genet. 1992 Oct;235(1):1-10. PMID:1435721 doi:10.1007/BF00286175
- ↑ Parsons CA, Tsaneva I, Lloyd RG, West SC. Interaction of Escherichia coli RuvA and RuvB proteins with synthetic Holliday junctions. Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5452-6. PMID:1608954 doi:10.1073/pnas.89.12.5452
- ↑ Tsaneva IR, Müller B, West SC. ATP-dependent branch migration of Holliday junctions promoted by the RuvA and RuvB proteins of E. coli. Cell. 1992 Jun 26;69(7):1171-80. PMID:1617728 doi:10.1016/0092-8674(92)90638-s
- ↑ Baharoglu Z, Petranovic M, Flores MJ, Michel B. RuvAB is essential for replication forks reversal in certain replication mutants. EMBO J. 2006 Feb 8;25(3):596-604. PMID:16424908 doi:10.1038/sj.emboj.7600941
- ↑ Shiba T, Iwasaki H, Nakata A, Shinagawa H. SOS-inducible DNA repair proteins, RuvA and RuvB, of Escherichia coli: functional interactions between RuvA and RuvB for ATP hydrolysis and renaturation of the cruciform structure in supercoiled DNA. Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8445-9. PMID:1833759 doi:10.1073/pnas.88.19.8445
- ↑ Le Masson M, Baharoglu Z, Michel B. ruvA and ruvB mutants specifically impaired for replication fork reversal. Mol Microbiol. 2008 Oct;70(2):537-48. PMID:18942176 doi:10.1111/j.1365-2958.2008.06431.x
- ↑ Iwasaki H, Shiba T, Makino K, Nakata A, Shinagawa H. Overproduction, purification, and ATPase activity of the Escherichia coli RuvB protein involved in DNA repair. J Bacteriol. 1989 Oct;171(10):5276-80. PMID:2529252 doi:10.1128/jb.171.10.5276-5280.1989
- ↑ Lloyd RG, Benson FE, Shurvinton CE. Effect of ruv mutations on recombination and DNA repair in Escherichia coli K12. Mol Gen Genet. 1984;194(1-2):303-9. PMID:6374379 doi:10.1007/BF00383532
- ↑ Parsons CA, West SC. Formation of a RuvAB-Holliday junction complex in vitro. J Mol Biol. 1993 Jul 20;232(2):397-405. PMID:8393934 doi:10.1006/jmbi.1993.1399
- ↑ Tsaneva IR, Muller B, West SC. RuvA and RuvB proteins of Escherichia coli exhibit DNA helicase activity in vitro. Proc Natl Acad Sci U S A. 1993 Feb 15;90(4):1315-9. PMID:8433990
- ↑ Hiom K, Tsaneva IR, West SC. The directionality of RuvAB-mediated branch migration: in vitro studies with three-armed junctions. Genes Cells. 1996 May;1(5):443-51. PMID:9078376 doi:10.1046/j.1365-2443.1996.d01-253.x
- ↑ Seigneur M, Bidnenko V, Ehrlich SD, Michel B. RuvAB acts at arrested replication forks. Cell. 1998 Oct 30;95(3):419-30. PMID:9814711 doi:10.1016/s0092-8674(00)81772-9
- ↑ van Gool AJ, Hajibagheri NM, Stasiak A, West SC. Assembly of the Escherichia coli RuvABC resolvasome directs the orientation of holliday junction resolution. Genes Dev. 1999 Jul 15;13(14):1861-70. PMID:10421637 doi:10.1101/gad.13.14.1861
- ↑ Eggleston AK, Mitchell AH, West SC. In vitro reconstitution of the late steps of genetic recombination in E. coli. Cell. 1997 May 16;89(4):607-17. PMID:9160752 doi:10.1016/s0092-8674(00)80242-1
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