Structural highlights
9e6h is a 4 chain structure with sequence from Maackia amurensis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Method: | Electron Microscopy, Resolution 2.84Å |
| Ligands: | BMA, CA, MAN, MN, NAG |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
MALS_MAAAM Sialic acid-binding lectin recognizing oligosaccharides containing terminal sialic acid linked via alpha-2,3 bond to penultimate galactose residues (PubMed:3350806). Binds the trisaccharide sequence Neu5Ac-alpha-2,3-Gal-beta-1,4-GlcNAc (PubMed:1985926). Binds fetuin when fully glycosylated but not when the high mannose-type glycans are removed, although the secondary structure is virtually unaffected by deglycosylation of the high mannose-type glycans (PubMed:26003537). The lectin activity may depend on the presence of a single GlcNAc attached to N-90 (PubMed:26003537).[1] [2] [3]
References
- ↑ Knibbs RN, Goldstein IJ, Ratcliffe RM, Shibuya N. Characterization of the carbohydrate binding specificity of the leukoagglutinating lectin from Maackia amurensis. Comparison with other sialic acid-specific lectins. J Biol Chem. 1991 Jan 5;266(1):83-8 PMID:1985926
- ↑ Kim BS, Hwang HS, Park H, Kim HH. Effects of selective cleavage of high-mannose-type glycans of Maackia amurensis leukoagglutinin on sialic acid-binding activity. Biochim Biophys Acta. 2015 Sep;1850(9):1815-21. PMID:26003537 doi:10.1016/j.bbagen.2015.05.011
- ↑ Wang WC, Cummings RD. The immobilized leukoagglutinin from the seeds of Maackia amurensis binds with high affinity to complex-type Asn-linked oligosaccharides containing terminal sialic acid-linked alpha-2,3 to penultimate galactose residues. J Biol Chem. 1988 Apr 5;263(10):4576-85 PMID:3350806
