9gyh

HEW Lysozyme with His 15 functionalized with iodoacetamide

spinqualitylabelsall models 9gyh, resolution 1.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9gyh is a 2 chain structure with sequence from Gallus gallus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.6Å Ligands: A1IQX, CL, NA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function LYSC_CHICK Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus. Publication Abstract from PubMed Hen egg-white lysozyme (HEWL) is a small polycationic protein which is highly soluble and stable. This has led to it becoming a ;molecular laboratory' where chemical biological operations and structural techniques are tested. To date, HEWL accounts for 1233 PDB entries, roughly 0.5% of the total, making it the best-represented protein in the PDB. With the aim of unambiguously identifying the N atom of the His15 side chain that is most reactive towards iodoacetamide, the structure of chemically modified HEWL was determined by crystallizing it using the ;15 minutes lysozyme' protocol. This protocol invariably yields tetragonal crystals of the unmodified protein. To our surprise, we found that the crystals of the modified protein had similar unit-cell parameters but that refinement was only possible when considering an orthorhombic system. The structure of His15 acetamide-modified hen egg-white lysozyme: a nice surprise from an old friend.,Malanho da Silva J, Lanuza J, Bruno F, Calderone V, Ravera E Acta Crystallogr F Struct Biol Commun. 2025 Feb 1;81(Pt 2):41-46. doi: , 10.1107/S2053230X2500010X. Epub 2025 Jan 13. PMID:39804568 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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