9h74

Late-stage 48S Initiation Complex with eIF3 (LS48S-eIF3 IC) guided by the trans-RNA

spinqualitylabelsall models 9h74, resolution 2.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9h74 is a 10 chain structure with sequence from Oryctolagus cuniculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 2.9Å Ligands: I2T, T6A Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function G1T2G4_RABIT Publication Abstract from PubMed Several approaches exist to silence genes, but few tools are available to activate individual mRNAs for translation inside cells. Guiding ribosomes to specific start codons without altering the original sequence remains a formidable task. Here we design capped trans-RNAs capable of directing ribosomes to specific initiation sites on individual mRNAs when the trans-cap is positioned near the target start codon. Structural and biochemical data suggest that the capped trans-RNA facilitates ribosome loading and scanning on the target mRNA through a synergistic mechanism involving alternative cap recognition. The trans-RNA also acts independently of the cap on the target mRNA, enabling translation of circular RNAs lacking internal ribosome entry sites. We apply trans-RNAs in vivo to achieve programmable alternative translation of endogenous genes in mouse liver. Finally, we provide the evidence for the existence of natural transcripts that, similarly to exogenous trans-RNAs, activate translation of endogenous mRNAs. Programmable initiation of mRNA translation by trans-RNA.,Jia L, Nguyen TT, Uematsu S, Gu Y, Shi S, Hashem Y, Qian SB Nat Biotechnol. 2025 Nov 21. doi: 10.1038/s41587-025-02897-1. PMID:41272315 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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